Japanese TOP page

logo
Scipas DX (Single-Charged Ion Peak Analyzing System) ver 1.39
This software helps researchers of protein H/D exchange using MALDI-MS.

H/D exchange analysis is difficult, because results have isotopic complexity. If you try to masure H/D exchange of proteins using MALDI-MS, this software would be usefull.

*"Scipas DX" is a free software. Please contact me(e-mail«) if you need anything.

Reference
Tatsuya Yamamoto, Tohru Yamagaki., Honoo Satake*.
gDevelopment of Software for the In-Depth Analysis of Protein Dynamics as Determined by MALDI Mass Spectrometry-Based Hydrogen/Deuterium Exchangeh
Mass Spectrom (Tokyo) , 8, S0082 (2019)

recent update:
2020.4.23 Graphical refinement. ver 1.39

"Download" –This software needs Java. Java Download Ë (English,Japanese)
Frames
This program has four frame: Spectrum main frame (SpectraManager), HD exchange result frame (HDX Manager), Sequence frame (Sequence Manager), and Profile frame of exchanged deouterium (Isotopic Imager).

four



How to operate?

  1. read sequence file (e.g. :"test_seq.prds") in sequence manager.

    2. squence


  2. select residues, start and end, of an analyze peptide in sequence manager.


  3. push "add buttonadd" in sequence manager. new peptide is added into HDX manager.

    4. HDX


  4. read spectra (e.g. :"test_spec.spe") file in spectra manager.

    5. graph


  5. push analysis


  6. finish

    7. *exchanged deuterium profile is drawn in Isotopic imager.
    graph



    *save data contained three files (e.g. :"test.hdx", "test_spec.spe", "test_seq.prds").
    *you can re-calculate only single peptide.graph

    *you can calculate all sequence files in a folder by one click., too.graph

    In analyzing time, you can drink coffee or do another experiment.

Up date log. (year.month.day)


Send me e-mail for Scipas DX to"mail".
Copyright (C) 2017- Tatsuya Yamamoto. All Rights Reserved.